7. Enzyme Inhibition and Regulation

During kinase phosphorylation, which phosphate group is removed from ATP in the figure below?

Covalent modification of an enzyme usually involves phosphorylation / dephosphorylation of:

When the active site of an enzyme is phosphorylated on one of its catalytic amino acid residues, the overall _______________ charge of phosphate groups would _____________ the affinity for a polar, negatively charged substrate.