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Multiple Choice
What is the function of the release factor during translation?
A
It binds to the stop codon in the A site in place of a tRNA.
B
It releases the amino acid from its tRNA to allow the amino acid to be added to the growing polypeptide.
C
It supplies a source of energy for termination of translation and the release of the polypeptide.
D
It releases the ribosome from the rough endoplasmic reticulum and allows the polypeptide to enter the cytosol.
Verified step by step guidance
1
Understand the process of translation: Translation is the process by which ribosomes synthesize proteins using mRNA as a template. It involves initiation, elongation, and termination phases.
Identify the role of the release factor: During the termination phase of translation, release factors are proteins that recognize stop codons on the mRNA strand.
Recognize the interaction with the ribosome: The release factor binds to the stop codon located in the A site of the ribosome, which is where tRNA usually binds during elongation.
Explain the mechanism of termination: Once the release factor is bound to the stop codon, it facilitates the release of the newly synthesized polypeptide chain from the tRNA in the P site.
Clarify the energy aspect: The release factor does not supply energy; rather, it catalyzes the hydrolysis of the bond between the polypeptide and tRNA, allowing the polypeptide to be released from the ribosome.