Describe the gene and protein defects in phenylketonuria (PKU). How are these defects connected to disease symptoms?

A portion of a DNA template strand has the base sequence
5′-...ACGCGATGCGTGATGTATAGAGCT...-3′
Identify the sequence and polarity of the mRNA transcribed from this fragmentary template-strand sequence.
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Polarity of Nucleic Acids
Base Pairing Rules
Several lines of experimental evidence pointed to a triplet genetic code. Identify three pieces of information that supported the triplet hypothesis of genetic code structure.
Outline the events that occur during initiation of translation in E. coli.
A portion of a DNA template strand has the base sequence
5′-...ACGCGATGCGTGATGTATAGAGCT...-3′
Assume the mRNA is written in the correct reading frame. Determine the amino acid sequence encoded by this fragment. Identify the N- and C-terminal directions of the polypeptide.
A portion of a DNA template strand has the base sequence
5′-...ACGCGATGCGTGATGTATAGAGCT...-3′
Which is the third amino acid added to the polypeptide chain?
Describe three features of tRNA molecules that lead to their correct charging by tRNA synthetase enzymes.
