Hershey and Chase selected the bacteriophage T2 for their experiment assessing the role of DNA in heredity because T2 contains protein and DNA, but not RNA. Explain why T2 was a good choice for this experiment.
Ch. 7 - DNA Structure and Replication

Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 7, Problem 4
Explain how the Hershey and Chase experiment identified DNA as the hereditary molecule.
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The Hershey and Chase experiment used bacteriophages, which are viruses that infect bacteria, to determine whether DNA or protein was the genetic material.
They labeled the DNA of the phages with radioactive phosphorus-32 (\(^{32}\text{P}\)) and the protein coat with radioactive sulfur-35 (\(^{35}\text{S}\)), as DNA contains phosphorus but not sulfur, and proteins contain sulfur but not phosphorus.
The labeled phages were allowed to infect bacteria, and then a blender was used to separate the phage coats from the bacterial cells.
After centrifugation, the bacteria formed a pellet at the bottom of the tube, while the phage coats remained in the supernatant.
They found that the radioactive phosphorus (\(^{32}\text{P}\)) was inside the bacterial cells, indicating that DNA was the material injected by the phages into the bacteria, while the radioactive sulfur (\(^{35}\text{S}\)) remained in the supernatant, indicating that proteins were not the genetic material.

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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Hershey and Chase Experiment
The Hershey and Chase experiment, conducted in 1952, utilized bacteriophages (viruses that infect bacteria) to determine whether DNA or protein was the genetic material. They labeled the DNA with radioactive phosphorus and the protein with radioactive sulfur, then tracked which component entered the bacterial cells. The results showed that only the DNA entered the cells, indicating that DNA is the hereditary molecule.
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Bacteriophages
Bacteriophages, or phages, are viruses that specifically infect bacteria. They consist of a protein coat and genetic material, which can be either DNA or RNA. In the Hershey and Chase experiment, phages were used as a model to study the transfer of genetic information, providing a clear system to distinguish between DNA and protein as the hereditary substance.
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Radioactive Labeling
Radioactive labeling is a technique used to trace the movement of molecules in biological experiments. In the Hershey and Chase experiment, the researchers used radioactive isotopes to label DNA and protein separately. This allowed them to track which component was responsible for carrying genetic information into the bacterial cells, ultimately demonstrating that DNA, not protein, was the hereditary material.
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Related Practice
Textbook Question
Textbook Question
What results from the experiments of Frederick Griffith provided the strongest support for his conclusion that a transformation factor is responsible for heredity?
Textbook Question
Explain why Avery, MacLeod, and McCarty's in vitro transformation experiment showed that DNA, but not RNA or protein, is the hereditary molecule.
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Textbook Question
One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.
What is the sequence of the other strand in the duplex?
Textbook Question
One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.
What is the name of the bond that joins one nucleotide to another in the DNA strand?
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Textbook Question
One strand of a fragment of duplex DNA has the sequence 5'-ATCGACCTGATC-3'.
Is the bond in part (b) a covalent or a noncovalent bond?
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