You have identified a gene encoding the protein involved in the rate-limiting step in vitamin E biosynthesis. How would you create a transgenic plant producing large quantities of vitamin E in its seeds?

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Hi, everyone. Let's look at our next question. What is the first step in creating a transgenic plant producing vitamin E in its seeds? Choice A identify the gene encoding the rate limiting enzyme in vitamin E biosynthesis. Choice B clone and isolate the gene from the plant. Choice C insert the gene into a plant transformation vector or choice D transform the plant cells with the gene containing vector. Well, this question though, at first, it might look a little complex is actually quite easy because we're the last for the first step. So putting these things in order and choice A has identified the gene as the step. Every other. Choice BC and D involves doing something with the gene for the rate limiting enzyme and vitamin E biosynthesis. We can't do anything with this gene until we've identified it. So that makes our choice of a quite clear if we want to just look at our other choices to be thorough here. So as we said, we first identified this gene and we're going to target what's called the rate limiting enzyme. As we recall, most reactions taking place in living organisms need enzymes to help them take place at a reasonable rate. So if you want to modify a plant to make it produce more vitamin E A promising target would be the rate limiting enzyme. You can modify that to turn up production. So once we've done that, we have our gene and then you would want to cologne and isolate the gene from the plant so that you can then use that to create transgenic plants that will produce more vitamin E. So B is not our answer choice, but that will kind of be the next step there. And then once we've got this isolated gene, we would want to insert the gene into a plant transformation vector. Choice C that can be used to insert a gene into another plant that doesn't have it in the first place. And finally choice d transform the plant cells with the gene containing vector. And then from there go on to grow transgenic plants that might produce vitamin E and large amounts in their seeds. But our first step to creating a transgenic plant would be choice a identify the gene encoding the rate limiting enzyme in vitamin E biosynthesis. See you in the next video.

You have identified a gene encoding the protein involved in the rate-limiting step in vitamin E biosynthesis. How would you create a transgenic plant producing large quantities of vitamin E in its seeds?

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Key Concepts

Transgenic Plants

Gene Expression Regulation

Metabolic Pathways