6. Enzymes and Enzyme Kinetics

Consider the equation for the line on the following Lineweaver-Burk plot: y = 6x + 3

A) What is the K_m for the corresponding enzyme?

a) -2 mM 

b) 18 mM 

c) 2 mM 

d) 9 mM

B) What is the V_max for the corresponding enzyme?

a) 0.333 mM/s 

b) 9 mM/s 

c) -6 mM/s 

d) 18 mM/s

To determine the V_max from a Lineweaver-Burk plot you would:

A Lineweaver-Burk plot generates a line with the following formula: y = 0.3x + 0.4. What is the K_m?

Suppose the data on the right is plotted onto a Lineweaver-Burk plot to obtain a straight line.

A) What is the value of the y-intercept of the line? ____________

B) What is the value of the x-intercept of the line? ____________