Match each of the following processes (1 to 5) with one of the items (a to e):
4. recombinant DNA
a. amino acids are linked together
b. RNA template is used to synthesize DNA
c. helicase unwinds DNA
d. genetic information is transferred from DNA
e. sticky ends join new DNA segment

Question

Match each of the following processes (1 to 5) with one of the items (a to e):

4. recombinant DNA

a. amino acids are linked together

b. RNA template is used to synthesize DNA

c. helicase unwinds DNA

d. genetic information is transferred from DNA

e. sticky ends join new DNA segment

Accepted Answer

All right. Hello everyone. So this question is asking us which of the given processes best explains the formation of recombinant DNA. So here we have four different and choices labeled A through D which we'll discuss in greater detail as we go through this video. But before we go ahead and do that, let's just talk briefly about recombinant DNA. Now recompete DNA is a technology or rather a technique that involves creating a new DNA sequence. So we're trying to create new DNA. And the question is how does that happen? Right? To understand how that happens, I want to draw your attention to the phrase recombinant. So what does that mean? Right. The word recombinant should bring to mind the word recombining. And so what we're doing here is we're taking DNA fragments, right? Or rather certain DNA sequences, we're breaking them apart and then we're recombining those fragments in different ways to produce a new DNA molecule. So that's the keyword. Here, we are recombining fragments of DNA to create a new sequence. So with this context in mind, let's go ahead and discuss our choices starting with option A option A says the joining of sticky ends using restriction enzymes. Now, this is actually accurate, right? Because recall that restriction enzymes are enzymes that cut DNA at a very specific sequence. But oftentimes these enzymes don't cut the DNA evenly. Instead, what results are the formation of these sticky ends? So DNA is two double stranded, right? And so after the restriction enzyme cuts, the DNA one strand could end up having an overhanging single stranded portion that overhanging part is described as the sticky end. And so for example, if we take two DNA fragments that each have sticky ends, these can be combined together to create a new sequence. So just sticking or combining those sticky ends together. Now this is going to be the correct answer. But for the sake of completeness, let's talk about the other choices starting with option B that says cutting DNA randomly with enzymes. Now here when talking about option A, we specifically mentioned how restriction enzymes are selective for one specific sequence. And this is because the technique of recombinant DNA requires a lot of precision precision, right. So it's in our best interest to use restriction enzymes so that we know exactly what part of the sequence has been cut. So option B is incorrect because cutting DNA randomly is hard to control and therefore not in our best interest from there, we move on to option C which says amplifying DNA through polymerase chain reaction or PCR. Now PC R is a technique used to make copies of a DNA segment or a DNA sequence. Now, this isn't to discount the importance of PC R in molecular biology, but it's not really describing recombinant DNA because we're not creating new DNA in PC R. We're taking one piece of DNA and making multiple copies of that piece. So nothing new is being created in the case of PC R. And now for option D which says transcribing DNA into MRN A, this is the process of transcription, which will eventually make important proteins after translation. Right now, transcription is certainly important in gene expression. But it's not really relevant in recombinant DNA. Because in recombinant DNA, we have to manipulate certain DNA molecules and create new sequences. So once again, option A is our correct answer and there you have it. So thank you so much for watching and I hope you found this helpful.

Match each of the following processes (1 to 5) with one of the items (a to e):
4. recombinant DNA
a. amino acids are linked together
b. RNA template is used to synthesize DNA
c. helicase unwinds DNA
d. genetic information is transferred from DNA
e. sticky ends join new DNA segment

Verified video answer for a similar problem:

Key Concepts

Recombinant DNA Technology

Sticky Ends

Gene Transfer

Match each of the following processes (1 to 5) with one of the items (a to e):4. recombinant DNAa. amino acids are linked togetherb. RNA template is used to synthesize DNAc. helicase unwinds DNAd. genetic information is transferred from DNAe. sticky ends join new DNA segment

Verified video answer for a similar problem:

Key Concepts

Recombinant DNA Technology

Sticky Ends

Gene Transfer

Match each of the following processes (1 to 5) with one of the items (a to e):
4. recombinant DNA
a. amino acids are linked together
b. RNA template is used to synthesize DNA
c. helicase unwinds DNA
d. genetic information is transferred from DNA
e. sticky ends join new DNA segment