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Ch.16 Amino Acids, Proteins, and Enzymes
Timberlake - Chemistry: An Introduction to General, Organic, and Biological Chemistry 13th Edition
Timberlake13th EditionChemistry: An Introduction to General, Organic, and Biological ChemistryISBN: 9780134421353Not the one you use?Change textbook
Chapter 16, Problem 58b

Fresh pineapple contains the enzyme bromelain that hydrolyzes peptide bonds in proteins.
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b. Fresh pineapple is used in a marinade to tenderize tough meat. Why?

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Fresh pineapple contains the enzyme bromelain, which is a proteolytic enzyme. Proteolytic enzymes are specialized in breaking down proteins by hydrolyzing peptide bonds.
Meat is primarily composed of muscle proteins, which contribute to its toughness. These proteins are held together by peptide bonds.
When fresh pineapple is used in a marinade, the bromelain enzyme interacts with the proteins in the meat, catalyzing the hydrolysis of peptide bonds. This breaks the proteins into smaller peptides and amino acids.
The breakdown of proteins reduces the structural integrity of the muscle fibers, making the meat softer and more tender.
This enzymatic action is why fresh pineapple is effective in tenderizing tough meat when used in a marinade.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Bromelain

Bromelain is a proteolytic enzyme found in fresh pineapple that breaks down protein molecules by hydrolyzing peptide bonds. This enzymatic activity is responsible for the tenderizing effect of pineapple on meat, as it helps to denature and digest the proteins, making the meat softer and easier to chew.

Protein Hydrolysis

Protein hydrolysis is a biochemical process where peptide bonds in proteins are broken down into smaller peptides or amino acids, often facilitated by enzymes like bromelain. This process is crucial in cooking and food preparation, as it alters the texture and digestibility of proteins, enhancing the overall quality of the dish.
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Acidic Hydrolysis Concept 1

Marinade

A marinade is a mixture, often acidic, used to soak foods such as meat before cooking to enhance flavor and tenderness. In the case of fresh pineapple, the marinade not only imparts a sweet and tangy flavor but also utilizes bromelain to tenderize the meat, making it more palatable and improving its texture.