Multiple Choice
Which of the following methods allow for the isolation of a single protein?
22. Techniques in Cell Biology
Isolation and Purification of Proteins
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Which of the following methods separates proteins by a charge to mass ratio?
A
Protein purification
B
Affinity chromatography
C
Gel Electrophoresis
D
Column chromatography
Verified step by step guidance1
Understand that the problem is asking about a method that separates proteins based on their charge to mass ratio.
Recall that proteins can be separated by various properties such as size, charge, and affinity to certain ligands.
Identify that Gel Electrophoresis is a technique that separates molecules based on their size and charge. In this method, proteins are placed in a gel and an electric current is applied, causing proteins to migrate through the gel.
Recognize that the speed and direction of protein migration in Gel Electrophoresis depend on the charge to mass ratio of the proteins. Proteins with different charge to mass ratios will move differently through the gel.
Conclude that Gel Electrophoresis is the method that separates proteins by their charge to mass ratio, distinguishing it from other methods like Affinity Chromatography or Column Chromatography, which separate based on different properties.
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