Table of contents

1. Overview of Cell Biology2h 49m
2. Chemical Components of Cells1h 14m
3. Energy1h 33m
4. DNA, Chromosomes, and Genomes2h 31m
5. DNA to RNA to Protein2h 31m
6. Proteins1h 36m
7. Gene Expression1h 42m
8. Membrane Structure1h 4m
- The Lipid Bilayer
  38m
- Membrane Proteins
  25m
9. Transport Across Membranes1h 52m
10. Anerobic Respiration1h 5m
11. Aerobic Respiration1h 11m
12. Photosynthesis52m
13. Intracellular Protein Transport2h 18m
14. Cell Signaling1h 28m
15. Cytoskeleton and Cell Movement1h 39m
16. Cell Division3h 5m
17. Meiosis and Sexual Reproduction50m
18. Cell Junctions and Tissues48m
19. Stem Cells13m
- Stem Cells
  13m
20. Cancer44m
21. The Immune System1h 6m
22. Techniques in Cell Biology1h 41m

22. Techniques in Cell Biology

Studying Proteins

Cell Biology

22. Techniques in Cell Biology

Studying Proteins

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Multiple Choice

Which method would be best to use if you wanted to identify unknown proteins?

SDS-PAGE

Mass Spec

NMR

Yeast two-hybrid test

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Verified step by step guidance

Understand the purpose of each method: SDS-PAGE is used for separating proteins based on their size, Mass Spectrometry (Mass Spec) is used for identifying and characterizing proteins, NMR is used for determining the structure of proteins, and the Yeast two-hybrid test is used for studying protein-protein interactions.

Consider the goal of the task: The task is to identify unknown proteins, which means you need a method that can provide information about the protein's identity, such as its amino acid sequence or molecular weight.

Evaluate the methods: SDS-PAGE can separate proteins but does not identify them. NMR provides structural information but is not typically used for identification. The Yeast two-hybrid test identifies interactions, not the proteins themselves.

Focus on Mass Spectrometry: Mass Spec is a powerful analytical technique that can determine the mass of a protein and provide detailed information about its amino acid sequence, making it ideal for identifying unknown proteins.

Conclude that Mass Spectrometry is the best choice for identifying unknown proteins due to its ability to provide precise molecular information and identify proteins based on their mass and sequence data.