Multiple Choice
Which of the following methods separates proteins by a charge to mass ratio?
22. Techniques in Cell Biology
Isolation and Purification of Proteins
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Which of the following methods allow for the isolation of a single protein?
A
Protein purification
B
Affinity chromatography
C
Gel Electrophoresis
D
Column chromatography
Verified step by step guidance1
Understand the goal of protein purification: The aim is to isolate a single type of protein from a complex mixture, which is crucial for studying its structure and function.
Learn about affinity chromatography: This method uses a specific ligand that binds to the target protein, allowing it to be separated from other proteins. The target protein is then eluted by changing the conditions (e.g., pH or ionic strength).
Explore gel electrophoresis: This technique separates proteins based on size and charge by applying an electric field to a gel matrix. While it can separate proteins, it is not typically used for isolating a single protein for further use.
Examine column chromatography: This method involves passing a protein mixture through a column filled with a stationary phase. Different proteins interact with the stationary phase to varying degrees, allowing for separation. However, it may require additional steps to isolate a single protein.
Conclude with the most suitable method: Affinity chromatography is often the most effective method for isolating a single protein due to its specificity and ability to selectively bind the target protein.
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