Multiple Choice
Which of the following shows the correct order of steps for PCR?
22. Techniques in Cell Biology
Polymerase Chain Reaction - PCR
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How many copies of DNA exist after 4 cycles of PCR?
A
4
B
8
C
16
D
32
Verified step by step guidance1
Understand the basic principle of PCR (Polymerase Chain Reaction), which is a technique used to amplify a segment of DNA, doubling the number of DNA copies with each cycle.
Start with a single copy of the DNA template. In the first cycle, the DNA is denatured, annealed with primers, and extended, resulting in 2 copies of DNA.
In the second cycle, each of the 2 DNA copies is again denatured, annealed, and extended, resulting in 4 copies of DNA.
In the third cycle, the 4 DNA copies are denatured, annealed, and extended, resulting in 8 copies of DNA.
In the fourth cycle, the 8 DNA copies are denatured, annealed, and extended, resulting in 16 copies of DNA. Thus, after 4 cycles, there are 16 copies of DNA.
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