22. Techniques in Cell Biology
DNA Cloning
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Which of the following shows the correct order of DNA cloning steps?
A
DNA extraction → Digestion → Place DNA into E. coli → DNA ligase
B
DNA ligase → Digestion → DNA extraction → Place DNA into E. coli
C
DNA extraction → Digestion → DNA ligase → Place DNA into E. coli
D
Digestion → DNA extraction → DNA ligase → Place DNA into E. coli
Verified step by step guidance1
Understand the process of DNA cloning, which involves several key steps to replicate DNA within a host organism.
Begin with DNA extraction, which is the process of isolating DNA from cells or tissues. This is the first step to obtain the DNA that will be cloned.
Next, perform digestion using restriction enzymes. These enzymes cut the DNA at specific sequences, creating fragments with 'sticky ends' that can be easily joined with other DNA fragments.
After digestion, use DNA ligase to join the DNA fragments. DNA ligase is an enzyme that facilitates the joining of DNA strands by forming phosphodiester bonds between the fragments.
Finally, introduce the ligated DNA into E. coli or another suitable host organism. This is typically done through a process called transformation, where the host cells take up the DNA and replicate it, allowing for the production of multiple copies of the DNA sequence.
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