Multiple Choice
How many copies of DNA exist after 4 cycles of PCR?
22. Techniques in Cell Biology
Polymerase Chain Reaction - PCR
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Which of the following shows the correct order of steps for PCR?
A
Melting DNA strands → Primer Binding → DNA polymerase replication
B
Primer Binding → Melting DNA strands → DNA polymerase replication
C
Melting DNA strands → DNA polymerase replication → Primer Binding
Verified step by step guidance1
Understand that PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences.
Recognize the three main steps of PCR: Denaturation, Annealing, and Extension.
Denaturation: Heat the DNA to around 94-98°C to separate the double-stranded DNA into single strands. This is often referred to as 'Melting DNA strands'.
Annealing: Cool the reaction to around 50-65°C to allow primers to bind to the single-stranded DNA. This step is known as 'Primer Binding'.
Extension: Raise the temperature to around 72°C for DNA polymerase to synthesize new DNA strands by adding nucleotides to the primers. This is the 'DNA polymerase replication' step.
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Polymerase Chain Reaction - PCR practice set
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