Table of contents

1. Overview of Cell Biology2h 49m
2. Chemical Components of Cells1h 14m
3. Energy1h 33m
4. DNA, Chromosomes, and Genomes2h 31m
5. DNA to RNA to Protein2h 31m
6. Proteins1h 36m
7. Gene Expression1h 42m
8. Membrane Structure1h 4m
- The Lipid Bilayer
  38m
- Membrane Proteins
  25m
9. Transport Across Membranes1h 52m
10. Anerobic Respiration1h 5m
11. Aerobic Respiration1h 11m
12. Photosynthesis52m
13. Intracellular Protein Transport2h 18m
14. Cell Signaling1h 28m
15. Cytoskeleton and Cell Movement1h 39m
16. Cell Division3h 5m
17. Meiosis and Sexual Reproduction50m
18. Cell Junctions and Tissues48m
19. Stem Cells13m
- Stem Cells
  13m
20. Cancer44m
21. The Immune System1h 6m
22. Techniques in Cell Biology1h 41m

22. Techniques in Cell Biology

Studying Proteins

Cell Biology

22. Techniques in Cell Biology

Studying Proteins

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Multiple Choice

Which method would be best to use if you wanted to analyze the structure of a protein?

SDS-PAGE

Mass Spec

NMR

Yeast two-hybrid test

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Verified step by step guidance

Understand the purpose of each method: SDS-PAGE is used for separating proteins based on their size, Mass Spectrometry (Mass Spec) is used for identifying the mass and composition of proteins, NMR (Nuclear Magnetic Resonance) is used for determining the 3D structure of proteins, and the Yeast two-hybrid test is used for studying protein-protein interactions.

Identify the goal of the analysis: The problem asks for a method to analyze the structure of a protein, which involves understanding the 3D conformation and spatial arrangement of atoms within the protein.

Evaluate the methods based on the goal: NMR is specifically designed to provide detailed information about the 3D structure of proteins in solution, making it suitable for structural analysis.

Consider the limitations and advantages: NMR can provide detailed structural information without the need for crystallization, unlike X-ray crystallography, and is suitable for proteins in their native environment.

Conclude that NMR is the best method for analyzing the structure of a protein, as it directly addresses the requirement of understanding the protein's 3D conformation.