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Multiple Choice
Green olives may be preserved in brine, which is a 30% salt solution. How does this method of preservation prevent microorganisms from growing in the olives?
A
Bacterial cells shrivel up in high salt solutions, causing the cell to burst.
B
High salt concentration lowers the pH, thus inhibiting bacterial metabolism.
C
High salt concentration raises the pH, thus inhibiting bacterial metabolism.
D
A 30% salt solution is hypotonic to the bacteria, so they gain too much water and burst.
E
A 30% salt solution is hypertonic to the bacteria, so they lose too much water and undergo plasmolysis.
Verified step by step guidance
1
Understand the concept of tonicity: Tonicity refers to the relative concentration of solutes in two fluids separated by a semipermeable membrane. A hypertonic solution has a higher concentration of solutes compared to the inside of a cell, while a hypotonic solution has a lower concentration.
Recognize the effect of hypertonic solutions on cells: In a hypertonic environment, water moves out of the cell to balance the solute concentration, leading to cell shrinkage or plasmolysis. This is because water moves from an area of lower solute concentration to an area of higher solute concentration.
Apply this concept to bacterial cells in brine: When bacterial cells are exposed to a 30% salt solution, the external environment is hypertonic compared to the inside of the bacterial cells. This causes water to move out of the bacterial cells.
Understand the consequences of water loss: As water exits the bacterial cells, they undergo plasmolysis, which is the process of cell shrinkage due to water loss. This inhibits bacterial growth and metabolism, effectively preserving the olives.
Conclude the preservation mechanism: The hypertonic nature of the brine solution prevents microorganisms from thriving by causing them to lose water and undergo plasmolysis, thus preserving the olives.