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Problem 1a
In the discussion, we focused on how DNA is replicated and synthesized. We also discussed recombination at the DNA level. Along the way, we encountered many opportunities to consider how this information was acquired. On the basis of these discussions, what answers would you propose to the following fundamental questions?
What is the experimental basis for concluding that DNA replicates semiconservatively in both bacteria and eukaryotes?
Problem 1b
In the discussion, we focused on how DNA is replicated and synthesized. We also discussed recombination at the DNA level. Along the way, we encountered many opportunities to consider how this information was acquired. On the basis of these discussions, what answers would you propose to the following fundamental questions?
How was it demonstrated that DNA synthesis occurs under the direction of DNA polymerase III and not polymerase I?
Problem 1c
In the discussion, we focused on how DNA is replicated and synthesized. We also discussed recombination at the DNA level. Along the way, we encountered many opportunities to consider how this information was acquired. On the basis of these discussions, what answers would you propose to the following fundamental questions?
How do we know that in vivo DNA synthesis occurs in the 5' to 3' direction?
Problem 1d
In the discussion, we focused on how DNA is replicated and synthesized. We also discussed recombination at the DNA level. Along the way, we encountered many opportunities to consider how this information was acquired. On the basis of these discussions, what answers would you propose to the following fundamental questions?
How do we know that DNA synthesis is discontinuous on one of the two template strands?
Problem 1e
In the discussion, we focused on how DNA is replicated and synthesized. We also discussed recombination at the DNA level. Along the way, we encountered many opportunities to consider how this information was acquired. On the basis of these discussions, what answers would you propose to the following fundamental questions?
What observations reveal that a 'telomere problem' exists during eukaryotic DNA replication, and how did we learn of the solution to this problem?
Problem 2
Write a short essay that distinguishes between the terms replication and synthesis, as applied to DNA. Which of the two is most closely allied with the field of biochemistry?
Problem 3
Compare conservative, semiconservative, and dispersive modes of DNA replication.
Problem 4
Describe the role of ¹⁵N in the Meselson–Stahl experiment.
Problem 5
Predict the results of the experiment by Taylor, Woods, and Hughes if replication were
(a) conservative and
(b) dispersive.
Problem 6
What are the requirements for in vitro synthesis of DNA under the direction of DNA polymerase I?
Problem 7
In Kornberg's initial experiments, it was rumored that he grew E. coli in Anheuser-Busch beer vats. (Kornberg was working at Washington University in St. Louis.) Why do you think this might have been helpful to the experiment?
Problem 8
How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?
Problem 9
Which characteristics of DNA polymerase I raised doubts that its in vivo function is the synthesis of DNA leading to complete replication?
Problem 10
Kornberg showed that nucleotides are added to the 3' end of each growing DNA strand. In what way does an exposed 3'-OH group participate in strand elongation?