What are the requirements for in vitro synthesis of DNA under the direction of DNA polymerase I?

Write a short essay that distinguishes between the terms replication and synthesis, as applied to DNA. Which of the two is most closely allied with the field of biochemistry?

Compare conservative, semiconservative, and dispersive modes of DNA replication.

Describe the role of ¹⁵N in the Meselson–Stahl experiment.

Predict the results of the experiment by Taylor, Woods, and Hughes if replication were (a) conservative and (b) dispersive.

In Kornberg's initial experiments, it was rumored that he grew E. coli in Anheuser-Busch beer vats. (Kornberg was working at Washington University in St. Louis.) Why do you think this might have been helpful to the experiment?

How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?

Which characteristics of DNA polymerase I raised doubts that its in vivo function is the synthesis of DNA leading to complete replication?

DNA polymerase III is the main DNA-synthesizing enzyme in bacteria. Describe how it carries out its role of elongating a strand of DNA.