An attribute of growth behavior of eight bacteriophage mutants (1 to 8) is investigated in experiments that establish coinfection by pairs of mutants. The experiments determine whether the mutants complement one another or fail to complement These eight mutants are known to result from point mutation. The results of the complementation tests are shown below.
Gene-mapping information identifies mutations 2 and 3 as the flanking markers in this group of genes. Assuming these mutations are on opposite ends of the gene map, determine the order of mutations in the region of the chromosome.