A wild-type culture of haploid yeast is exposed to ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies (colonies numbered 1 to 6) are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid4 (replica plates numbered 1 to 4) with the following results:
Identify the colonies that are auxotrophic (mutant). What growth information leads to your answer?
Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Bacterial Transformation
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The DNA from a bacterium with the genotype a+ b+ c+ is used to transform a bacteria with the genotype a b c. Gene pairs were checked for cotransformation with the following results. Using these results determine which genes are linked.

A
A and B
B
B and C
C
A and C

1
Understand the concept of cotransformation: Cotransformation occurs when two genes are close enough on the DNA that they are transferred together during transformation. This indicates linkage between the genes.
Examine the data provided in the table: The table shows the results of cotransformation tests for gene pairs A and B, B and C, and A and C.
Interpret the results: The table indicates that cotransformation occurs for the gene pair A and B, but not for B and C or A and C.
Determine linkage: Since cotransformation is observed for A and B, these genes are likely linked, meaning they are close together on the DNA. The lack of cotransformation for B and C and A and C suggests these pairs are not linked.
Conclude which genes are linked: Based on the cotransformation results, the genes A and B are linked, while B and C and A and C are not linked.
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