If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?

Define plaque, lysogeny, and prophage.

Two theoretical genetic strains of a virus (a⁻b⁻c⁻ and a⁺b⁺c⁺) were used to simultaneously infect a culture of host bacteria. Of 10,000 plaques scored, the following genotypes were observed. Determine the genetic map of these three genes on the viral chromosome. Decide whether interference was positive or negative.a⁺ b⁺ c⁺ 4100 a⁻ b⁺ c⁻ 160a⁻ b⁻ c⁻ 3990 a⁺ b⁻ c⁺ 140a⁺ b⁻ c⁻ 740 a⁻ b⁻ c⁺ 90a⁻ b⁺ c⁺ 670 a⁺ b⁺ c⁻ 110

The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?

If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?Dilution Factor Assay Results(a) 10⁴ All bacteria lysed(b) 10⁵ 14 plaques(c) 10⁶ 0 plaques

In recombination studies of the rII locus in phage T4, what is the significance of the value determined by calculating phage growth in the K12 versus the B strains of E. coli following simultaneous infection in E. coli B? Which value is always greater?

In an analysis of rII mutants, complementation testing yielded the following results:Mutants Results (+/- lysis)1, 2 +1, 3 +1, 4 -1, 5 -Predict the results of testing 2 and 3, 2 and 4, and 3 and 4 together.

If further testing of the mutations in Problem 18 yielded the following results, what would you conclude about mutant 5?Mutants Results2, 5 -3, 5 -4, 5 -