Open QuestionYou have discovered a new species of archaea from a hot spring in Yellowstone National Park.How would your strategy change if you were unable to grow the strain in culture?
Open QuestionYou have discovered a new species of archaea from a hot spring in Yellowstone National Park.After growing a pure culture of this organism, what strategy might you employ to sequence its genome?
Open QuestionIn this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?How can we correlate the genome with RNA expression data in a tissue or a single cell?
Open QuestionIn this chapter, we focused on the analysis of genomes, transcriptomes, and proteomes and considered important applications and findings from these endeavors. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?How do we know which contigs are part of the same chromosome?
Open QuestionRepetitive DNA poses problems for genome sequencing.What types of repetitive DNA are most problematic?
Open QuestionWhen the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs.How can physical gaps be closed?
Open QuestionWhen the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs.What is the difference between physical and sequence gaps?