In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?How can we correlate the genome with RNA expression data in a tissue or a single cell?

Which of the following sequence techniques requires the use of vectors?

Dideoxy nucleotides (ddNTPs) are used in Sanger sequencing because they have what function?

In this chapter, we focused on the analysis of genomes, transcriptomes, and proteomes and considered important applications and findings from these endeavors. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?

How do we know which contigs are part of the same chromosome?