Open QuestionList the order in which the following proteins and enzymes are active in E. coli DNA replication: DNA pol I, SSB, ligase, helicase, DNA pol III, and primase.
Open QuestionTwo viral genomes are sequenced, and the following percentages of nucleotides are identified:Genome 1:A=28%,C=22%,G=28%,T=22%Genome 2:A=22%,C=28%,G=28%,T=22%Are the DNA molecules in each genome single stranded or double stranded?
Open QuestionA genetics student was asked to draw the chemical structure of an adenine- and thymine-containing dinucleotide derived from DNA. The answer is shown here:The student made more than six major errors. One of them is circled, numbered 1, and explained. Find five others. Circle them, number them 2 through 6, and briefly explain each in the manner of the example given.
Open QuestionOne of the most common spontaneous lesions that occurs in DNA under physiological conditions is the hydrolysis of the amino group of cytosine, converting the cytosine to uracil. What would be the effect on DNA structure of a uracil group replacing cytosine?
Open QuestionIn some organisms, cytosine is methylated at carbon 5 of the pyrimidine ring after it is incorporated into DNA. If a 5-methyl cytosine molecule is then hydrolyzed, as described in Problem 28, what base will be generated?
Open QuestionConsider the drawing of a dinucleotide below. Suppose that the molecule was cleaved with the enzyme spleen phosphodiesterase, which breaks the covalent bond connecting the phosphate to C-5'. After cleavage, to which nucleoside is the phosphate now attached (A or T)?