Multiple Choice
During a serial dilution, you use 15.0 mL of a standard solution and dilute it to 1.35 L twice. What is the final concentration if the standard solution had an initial concentration of 0.500 M?
6. Chemical Quantities & Aqueous Reactions
Dilutions
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Which set of serial dilution plates demonstrates a successful dilution process?
A
Plates showing an increase in colony count from 10^1 to 10^6 dilutions
B
Plates with no colonies present at any dilution level
C
Plates showing a gradual decrease in colony count from 10^1 to 10^6 dilutions
D
Plates with the same colony count across all dilution levels
Verified step by step guidance1
Understand the concept of serial dilution: Serial dilution involves stepwise dilution of a substance in solution, usually by a constant factor, to achieve a range of concentrations.
Recognize the expected outcome of a successful serial dilution: As the dilution factor increases, the concentration of the substance decreases, leading to fewer colonies on the plates.
Analyze the given options: A successful dilution process should show a gradual decrease in colony count as the dilution factor increases from 10^1 to 10^6.
Evaluate the options: Plates showing an increase in colony count or no colonies at all do not demonstrate a successful dilution. Similarly, plates with the same colony count across all dilutions indicate no change in concentration.
Conclude that the correct demonstration of a successful dilution process is plates showing a gradual decrease in colony count from 10^1 to 10^6 dilutions.
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