Multiple Choice
What is the main purpose of creating serial dilutions of a stock solution?
6. Chemical Quantities & Aqueous Reactions
Dilutions
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Why is it necessary to dilute a bacterial culture when plating for a counting experiment?
A
To increase the growth rate of bacteria on the plate.
B
To ensure that individual colonies can be counted accurately.
C
To enhance the nutrient availability for bacteria.
D
To prevent contamination from other microorganisms.
Verified step by step guidance1
Understand the purpose of diluting a bacterial culture: The main goal is to achieve a concentration of bacteria that allows individual colonies to grow separately on the agar plate.
Consider the concept of colony formation: When bacteria are spread on an agar plate, each viable bacterium can grow into a visible colony. If the culture is too concentrated, colonies will overlap, making it difficult to count them accurately.
Learn about the dilution process: Dilution involves mixing a small amount of bacterial culture with a larger volume of sterile medium, reducing the concentration of bacteria.
Recognize the importance of accurate counting: By diluting the culture, you ensure that the colonies are well-separated, which is crucial for obtaining an accurate count of the number of bacteria present in the original sample.
Understand the implications of dilution: Proper dilution helps avoid errors in counting due to overlapping colonies and ensures reliable results in experiments that require precise bacterial quantification.
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