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Problem 1a
How do we know that promoter and enhancer sequences control the initiation of transcription in eukaryotes?
Problem 1b
How do we know that the orientation of promoters relative to the transcription start site is important while enhancers are orientation independent?
Problem 1c
How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?
Problem 1d
How do we know that there is an association between disease susceptibility in humans and regulatory DNA sequences?
Problem 2
Write a short essay describing how cis-acting regulatory elements, activators, and chromatin modifiers are all coordinately involved in regulating transcription initiation.
Problem 3
What features of eukaryotes provide additional opportunities for the regulation of gene expression compared to bacteria?
Problem 4
Provide a definition of chromatin remodeling, and give two examples of this phenomenon.
Problem 5
Describe the organization of the interphase nucleus. Include in your presentation a description of chromosome territories, interchromatin compartments, and transcription factories.
Problem 6
A number of experiments have demonstrated that areas of the genome that are transcriptionally inactive are also resistant to DNase I digestion. However, transcriptionally active areas are DNase I sensitive. Describe how DNase I resistance or sensitivity might indicate transcriptional activity.
Problem 7
Provide a brief description of two different types of histone modification and how they impact transcription.
Problem 8
Present an overview of the manner in which chromatin can be remodeled. Describe the manner in which these remodeling processes influence transcription.