Which of the following terms is associated with closed chromatin?

DNA methylation is commonly associated with a reduction of transcription. The following data come from a study of the impact of the location and extent of DNA methylation on gene activity in eukaryotic cells. A bacterial gene, luciferase, was inserted into plasmids next to eukaryotic promoter fragments. CpG sequences, either within the promoter and coding sequence (transcription unit) or outside of the transcription unit, were methylated to various degrees, in vitro. The chimeric plasmids were then introduced into cultured cells, and luciferase activity was assayed. These data compare the degree of expression of luciferase with differences in the location of DNA methylation [Irvine et al. (2002). Mol. and Cell. Biol. 22:6689–6696]. What general conclusions can be drawn from these data? 

Chromosomal regions that form heterochromatin contain:

Which of the following are examples of epigenetic marks?

CpG islands are defined as which of the following?

In this chapter, we focused on epigenetic modifications to the genome that regulate gene expression. Several mechanisms are involved, and epigenetic control of gene expression is important in development, cancer, and modulating the genomic response to environmental factors. From the explanations given in the chapter,

How does an environmental factor like stress generate a response that is transmitted from generation to generation?

In this chapter, we focused on epigenetic modifications to the genome that regulate gene expression. Several mechanisms are involved, and epigenetic control of gene expression is important in development, cancer, and modulating the genomic response to environmental factors. From the explanations given in the chapter,

What is the evidence that epigenetic changes are involved in cancer?

In this chapter, we focused on epigenetic modifications to the genome that regulate gene expression. Several mechanisms are involved, and epigenetic control of gene expression is important in development, cancer, and modulating the genomic response to environmental factors. From the explanations given in the chapter,

How do we know how methylation of promoters silences gene expression?

In this chapter, we focused on how eukaryotic gene expression is regulated posttranscriptionally. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter:

How do we know that microRNAs negatively regulate target mRNAs?