Multiple Choice
The purpose of positional cloning is to what?
4. Genetic Mapping and Linkage
Positional Cloning
Struggling with Genetics?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Which of the following is a crucial step of positional cloning?
A
Sequencing the genome
B
Chromosomal walking
C
Identifying microsatellites
D
Generating RFLPs
Verified step by step guidance1
Understand the concept of positional cloning: Positional cloning is a method used to identify genes based on their location on a chromosome. It is often used when the gene product is unknown, but the gene's location is suspected based on linkage analysis.
Learn about chromosomal walking: Chromosomal walking is a technique used in positional cloning to move step-by-step along a chromosome from a known location to an unknown gene of interest. This involves using overlapping DNA fragments to 'walk' along the chromosome.
Recognize the role of chromosomal walking in positional cloning: Chromosomal walking is crucial because it allows researchers to narrow down the region of interest on a chromosome, eventually leading to the identification of the gene responsible for a particular trait or disorder.
Differentiate between chromosomal walking and other techniques: While sequencing the genome, identifying microsatellites, and generating RFLPs are important genetic techniques, chromosomal walking is specifically crucial for the step-by-step approach needed in positional cloning.
Apply this understanding to the problem: Given the options, identify that chromosomal walking is the crucial step in positional cloning, as it directly facilitates the identification of genes based on their chromosomal position.
Related Videos
Related Practice
Positional Cloning practice set
Problem sets built by lead tutorsExpert video explanations