Table of contents

1. Introduction to Genetics51m
2. Mendel's Laws of Inheritance3h 37m
3. Extensions to Mendelian Inheritance2h 41m
4. Genetic Mapping and Linkage2h 28m
5. Genetics of Bacteria and Viruses1h 21m
6. Chromosomal Variation1h 48m
7. DNA and Chromosome Structure56m
8. DNA Replication1h 10m
9. Mitosis and Meiosis1h 34m
10. Transcription1h 0m
11. Translation58m
12. Gene Regulation in Prokaryotes1h 19m
13. Gene Regulation in Eukaryotes44m
14. Genetic Control of Development44m
15. Genomes and Genomics1h 50m
16. Transposable Elements47m
17. Mutation, Repair, and Recombination1h 6m
18. Molecular Genetic Tools19m
- Genetic Cloning
  9m
- Methods for Analyzing DNA
  9m
19. Cancer Genetics29m
- Overview of Cancer
  21m
- Cancer Mutations
  7m
20. Quantitative Genetics1h 26m
21. Population Genetics50m
- Hardy Weinberg
  19m
- Allelic Frequency Changes
  31m
22. Evolutionary Genetics29m

18. Molecular Genetic Tools

Methods for Analyzing DNA

Genetics

18. Molecular Genetic Tools

Methods for Analyzing DNA

Previous problemNext problem

Struggling with Genetics?

Join thousands of students who trust us to help them ace their exams!Watch the first video

Multiple Choice

If you had a sample of RNA to analyze, which of the following techniques would you most likely use?

Southern Blots

SDS-PAGE Blots

Northern Blots

Western Blots

Previous problemNext problem

Verified step by step guidance

Understand the purpose of each blotting technique: Southern blotting is used for DNA analysis, Northern blotting is used for RNA analysis, Western blotting is used for protein analysis, and SDS-PAGE is a technique for separating proteins based on their size.

Identify the type of molecule you are analyzing: In this problem, you are analyzing RNA.

Match the molecule type to the appropriate technique: Since you are analyzing RNA, Northern blotting is the correct technique to use.

Consider why Northern blotting is suitable: Northern blotting allows for the detection and quantification of specific RNA sequences, making it ideal for RNA analysis.

Review the other options to confirm: Southern blots are for DNA, Western blots are for proteins, and SDS-PAGE is for protein separation, none of which are suitable for RNA analysis.

7:40m

Watch next

Master Methods for Analyzing DNA and RNA with a bite sized video explanation from Kylia

Start learning

Related Videos

Related Practice

Open Question

The electrophoresis gel shown in part (a) is from a DNase I footprint analysis of an operon transcription control region. DNA sequence analysis of a 35-bp region is shown in part (b). The control region, labeled with ³²P at one end, is shown in a map in part (c). Separate samples of control-region DNA are exposed to DNase I, and the resulting DNase I–digested DNA is run in separate lanes of the electrophoresis gel. Unprotected DNA is in lane 1, DNA protected by repressor protein is in lane 2, and RNA polymerase–protected DNA is in lane 3. The numbers along the electrophoresis gel correspond to the 35-bp sequence labeled on the map in part (c). Use the information provided to solve the following problems.Locate the regions of the sequence protected by repressor protein and by RNA polymerase.

Open Question

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.Identify the template and nontemplate strands of DNA.

Open Question

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.Write the DNA sequence of both alleles, including strand polarity.

Multiple Choice

In terms of molecular biology, what is a library?

Open Question

What is CODIS? Describe the four most important features of genetic markers used in this system.

Open Question

In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?From GWAS how do we know which genes are associated with a particular genetic disorder?

Open Question

In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?

How does a positive ASO test for sickle-cell anemia determine that an individual is homozygous recessive for the mutation that causes sickle-cell anemia?

Open Question

Compare and contrast the terms Paternity Index (PI) and Combined Paternity Index (CPI). How does each contribute to paternity determination?

Show more practices

Methods for Analyzing DNA practice set

Problem sets built by lead tutors

Expert video explanations

Go to Practice

Download the Mobile app

Privacy

Do not sell my personal information

Accessibility

Patent notice

Sitemap