Define and indicate the significance of (a) Okazaki fragments, (b) DNA ligase, and (c) primer RNA during DNA replication.

Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.

If mutation inactivated DNA polymerase I in a strain of E. coli, would the cell be able to replicate its DNA? If so, what kind of abnormalities would you expect to find in the cell?

Diagram a replication fork in bacterial DNA and label the following structures or molecules.

a. DNA pol III
b. helicase
c. RNA primer
d. origin of replication
e. leading strand (label its polarity)
f. DNA pol I
g. topoisomerase
h. SSB protein
i. lagging strand (label its polarity)
j. primase
k. Okazaki fragment