In enhancer trapping experiments, a minimal promoter and...

Question

In enhancer trapping experiments, a minimal promoter and a reporter gene are placed adjacent to the end of a transposon so that genomic enhancers adjacent to the insertion site can act to drive expression of the reporter gene. In a modification of this approach, a series of enhancers and a promoter can be placed at the end of a transposon so that transcription is activated from the transposon into adjacent genomic DNA. What types of mutations do you expect to be induced by such a transposon in a mutagenesis experiment?

Accepted Answer

Hey, everyone. Let's take a look at this question together. What type of trans posible element has the ability to alter a gene or its regulatory region? Is it? Answer choice. A DNA transposon answer choice B retro transposon, answer choice C short interspersed nuclear element or answer choice D long interspersed nuclear elements. Let's work this problem out together to try to figure out what type of transposes element has the ability to alter a gene or its regulatory region. So we are looking for a trans posible element which is also called a transposon. And we are looking for a transposon that has the ability to alter gene or its regulatory region, meaning that the transposon affects the coding region. And so looking at our answer choices, we can see that answer choice. A DNA trans bosons, which we know that DNA trans bosons are trans bosons that affect the coding region by inserting themselves into the DNA sequence which allows them to alter the gene or its regulatory region. So answer choice. A DNA transposon is the correct answer. And we note that answer choice B retro transposon is incorrect since retro transposon insert themselves into the noncoding region and do not have the ability to alter a gene or its regulatory region. And we know that answer choice c short interspersed nuclear elements and answer choice d long interspersed nuclear elements are also incorrect. Since both of these are a type of retro transposon, meaning that they also do not have the ability to alter a gene or its regulatory region. And answer choice. A DNA transposon is the only type of transposes element here that has that ability, making answer choice. A the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

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