Following the tragic shooting of 20 children at a school in Newtown, Connecticut, in 2012, Connecticut's state medical examiner requested a full genetic analysis of the killer's genome. What do you think investigators might be looking for? What might they expect to find? Might this analysis lead to oversimplified analysis of the cause of the tragedy?

You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed using the vector shown in Problem 18. The cDNA was directionally cloned using the EcoRI and XhoI sites. You sequence the recombinant plasmid using primers complementary to the T7 and T3 promoter sites flanking the MCS (the positions of these sequences are shown in the figure in Problem 18). The first 30 to 60 bases of sequence are usually discarded since they tend to contain errors.Can you identify the start of the coding region in the end of the gene? What does the sequence preceding the start codon represent?

You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed using the vector shown in Problem 18. The cDNA was directionally cloned using the EcoRI and XhoI sites. You sequence the recombinant plasmid using primers complementary to the T7 and T3 promoter sites flanking the MCS (the positions of these sequences are shown in the figure in Problem 18). The first 30 to 60 bases of sequence are usually discarded since they tend to contain errors.Can you identify which sequence portions are derived from the vector (specifically the MCS) and which are derived from the cDNA clone?

You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed using the vector shown in Problem 18. The cDNA was directionally cloned using the EcoRI and XhoI sites. You sequence the recombinant plasmid using primers complementary to the T7 and T3 promoter sites flanking the MCS (the positions of these sequences are shown in the figure in Problem 18). The first 30 to 60 bases of sequence are usually discarded since they tend to contain errors.Will the long stretch of T residues in the T3 sequence exist in the genomic sequence of the gene?

In Drosophila, loss-of-function Ultrabithorax mutations result in the posterior thoracic segments differentiating into body parts with an identity normally found in the anterior thoracic segments. When the Ultrabithorax gene was cloned, it was shown to encode a transcription factor and to be expressed only in the posterior region of the thorax. Thus, Ultrabithorax acts to specify the identity of the posterior thoracic segments. Similar genes were soon discovered in other animals, including mice and humans. You have found that mice possess two closely related genes, Hoxa7 and Hoxb4, which are orthologs (see Genetic Analysis 14.2 for definition) of Ultrabithorax. You wish to know whether the two mouse genes act to specify the identity of body segments in mice.How will you determine where and when the mouse genes are expressed?

Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner typical of eukaryotic genomes, with 145 bp encircling each nucleosome and approximately 55 bp in linker DNA. When C. elegans chromatin is carefully isolated, stripped of nonhistone proteins, and placed in an appropriate buffer, the chromatin decondenses to the 10-nm fiber structure. Suppose researchers mix a sample of 10-nm–fiber chromatin with a large amount of the enzyme DNase I that randomly cleaves DNA in regions not protected by bound protein. Next, they remove the nucleosomes, separate the DNA fragments by gel electrophoresis, and stain all the DNA fragments in the gel.How do the expected results support the 10-nm–fiber model of chromatin?

Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner typical of eukaryotic genomes, with 145 bp encircling each nucleosome and approximately 55 bp in linker DNA. When C. elegans chromatin is carefully isolated, stripped of nonhistone proteins, and placed in an appropriate buffer, the chromatin decondenses to the 10-nm fiber structure. Suppose researchers mix a sample of 10-nm–fiber chromatin with a large amount of the enzyme DNase I that randomly cleaves DNA in regions not protected by bound protein. Next, they remove the nucleosomes, separate the DNA fragments by gel electrophoresis, and stain all the DNA fragments in the gel.Explain the origin of DNA fragments seen in the gel.