Multiple Choice
Which of the following terms describes the smallest distance that you can tell two objects apart?
22. Techniques in Cell Biology
The Light Microscope
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Which type of fluorescence microscopy can visualize two interacting proteins in a cell?
A
FRAP
B
FRET
C
FREP
D
FRAT
Verified step by step guidance1
Understand the context: The problem is asking about a type of fluorescence microscopy that can visualize interactions between two proteins within a cell.
Identify the key concept: Fluorescence Resonance Energy Transfer (FRET) is a technique used to study the interaction between two proteins. It involves energy transfer between two light-sensitive molecules (fluorophores) when they are in close proximity.
Differentiate between the options: FRAP (Fluorescence Recovery After Photobleaching) is used to study the movement and diffusion of proteins within cell membranes, not interactions. FREP and FRAT are not standard terms in fluorescence microscopy.
Focus on FRET: In FRET, when the donor fluorophore is excited, it can transfer energy to an acceptor fluorophore if they are close enough, indicating a protein-protein interaction.
Conclude with the correct choice: Based on the explanation, FRET is the appropriate technique for visualizing interactions between two proteins in a cell.
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