Multiple Choice
What type of mutant is necessary to study lethal mutants?
22. Techniques in Cell Biology
Genetic Screens
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Which test can be used to identify if two similar phenotypes in two different organisms are caused by mutations in the same gene?
A
Genetic Screen
B
Complementation Test
C
Epistasis Analysis
Verified step by step guidance1
Understand the concept of a complementation test: It is used to determine if two mutations that produce a similar phenotype are in the same gene or in different genes.
Identify the organisms or strains with the similar phenotypes that you want to test. These should be organisms that are homozygous for the mutations.
Cross the two organisms with the similar phenotypes. This involves mating the two organisms to produce offspring.
Observe the phenotype of the offspring. If the offspring display the wild-type phenotype, the mutations complement each other, indicating that the mutations are in different genes.
If the offspring display the mutant phenotype, the mutations do not complement each other, suggesting that the mutations are in the same gene.
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