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Multiple Choice
What is the ratio of HCO₃⁻ to H₂CO₃ in blood with a pH of 7.4?
A
20:1
B
5:1
C
1:1
D
10:1
Verified step by step guidance
1
Understand that the problem involves the bicarbonate buffer system in blood, which consists of HCO₃⁻ (bicarbonate ion) and H₂CO₃ (carbonic acid). The Henderson-Hasselbalch equation is used to relate the pH to the ratio of these components.
Recall the Henderson-Hasselbalch equation: . This equation will help us find the ratio of HCO₃⁻ to H₂CO₃.
Identify the pKₐ value for carbonic acid (H₂CO₃), which is approximately 6.1. This value is crucial for applying the Henderson-Hasselbalch equation.
Substitute the given pH value (7.4) and the pKₐ value (6.1) into the Henderson-Hasselbalch equation: . Solve for the log term to find the ratio.
Calculate the antilog of the result from the previous step to determine the ratio of HCO₃⁻ to H₂CO₃. This will give you the ratio that corresponds to the pH of 7.4.